The effect of calcium binding agents on the virulence of Clostridium perfringens for the white mouse.
نویسندگان
چکیده
Clostridium perfringens type A produces a variety of toxins. It has been shown that the alpha toxin (lecithinase) produced by this organism apparently plays a major role in its virulence (Evans, 1947) using the guinea pig as the test animal. If lecithinase is necesary for virulence, the organisms should be avirulent when the lecithinase is inhibited. Calcium ion is necessary for lecithinase activity, and addition of appropriate amounts of calcium binding agents will effectively inhibit this activity in vitro. The present paper is concerned with the effect of simultaneously injecting calcium binding agents and C. perfringen organisms in the white mouse. Although frequently used in gas gangrene studies, the white mouse is considerably more resistant to infection with C. pefringens than is the guinea pig. Some method of producing preliminary tissue breakdown is generally employed for initiating C. perfringens infection in the mouse. Two of the more frequently encountered methods of causing tissue breakdown are the use of high concentrations of ionizable calcium salts (Bullock and Cramer, 1919) or toxic culture filtrates (deKruif and Bolhann, 1917) as suspending agents for the organisms. In the course of the present study, it has been observed that washed C. perfringen organisms injected subcutaneously in comparatively large numbers are capable of causing the death of the white mouse, in the absence of any added tissue damaging agent.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 69 2 شماره
صفحات -
تاریخ انتشار 1955